Coordinator: Dean's Office
Elective Graduate Course
Dr. Narendra Deshmukh and Dr. Kalpana Kharade
Coordinator: Dean's Office
Elective Graduate Course
Prof. Sanjay Chandrasekharan & Dr. Sweta Anantharaman
Ms. Pranshi Upadhyay and Mr. Joseph Salve
03Mon
Coordinator: Dean's Office
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Dr. Tanuja Kistwal
Dr. Tanuja Kistwal has obtained her doctoral degree in the field of Fluorescence dynamics and microscopy of protein-based biomaterials and nanomaterials using time resolved spectroscopy and microscopy from the Indian Institute of Technology (IIT), Bombay, India, under the supervision of Prof. Anindya Datta.
Fluorescence dynamics and microscopy of protein-based biomaterials and Nanomaterials
The Excited state dynamics of biological phenomena have been studied using time resolved fluorescence techniques, Time-Correlated Single-Photon count (TCSPC), and Femtosecond Optical Gating (FOG). These techniques are based upon the determination of fluorescence lifetime and are useful for the determination of rates of radiative and nonradiative excited state processes. Microscopic measurements including fluorescence lifetime imaging and microscopy (FLIM), and fluorescence correlation spectroscopy (FCS), provide insights into the dynamics of biological processes and phenomena in restricted compartments. Solvent-free liquid protein that shows slow solvation, associated classically with biological water, has been observed in these systems, even in the waterless condition. This apparently intriguing observation has been rationalized by the relaxation of segments of the protein and the PS in the microenvironment of the fluorescent probe slow down the hydration dynamics of a protein and such slow dynamics is attributed to the chain dynamics of the protein, aggregates surfactant-fluorogenic aggregates that are found to disrupt protein fibrils along with white light generation and protein corona on a water-soluble nanoparticle.